Abstract:
Yeast cells were depleted of glutathione by treatment with iodoacetamide and were markedly more sensitive to damage caused by free radicals generated by AAPH (2,2 f-azobis(2-amidinopropane) dihydrochloride) and H^Oi- However intrinsic tolerance to heat and ethanol were not affected. Glutathione depletion inhibited the induction of tolerance to all four stresses and the induction of heat shock protein (hsp) synthesis. It was concluded that glutathione was essential for oxidative stress tolerance and induction of tolerance to oxidative, heat and ethanol stresses.
